SBRC Robots

Robotics

The SBRC-Nottingham robotic suite is a custom made system that consist of two interconnected robotic platforms that include:

  • Two Biomek FXp dual arm liquid handling instrument with multichannel 96-head and Span-8
  • 2D and 1D barcode readers
  • Pin Tool for microbial colony transfer
  • Integrated T-Robot thermal-cycler, Shaking and peltier devices
  • SCARA robotic arm and robotic shuttles that link all integrated devices
  • Molecular Devices Q-Pix 420 HT for high throughput colony picking up to 2000 colonies per hour plus bacterial spreading applications on omnitrays 
  • Solid Phase Extraction (SPE) system and magnetic based DNA purification system
  • Integrated Cytomat 2 shaker incubators and Cytomat plate hotels  
  • BigNeat enclose to ensure sterile environment
  • Molecular Devices SpectraMax i3 multi-mode plate reader 
  • High-throughput electroporation system (BTX™ Gemini X2 HT Electroporation System)
  • High-throughput gel electrophoresis system  

The instruments within both platforms can work in standalone and integrated modes. Our robotic system allows for the setup of customized protocols and workflows. It gives a wide range of options for gene assembly, microbial transformation (chemical and HT electroporation), colony plating and picking, transformant library screening, purification of nucleic acid, proteins and analytes, size section of DNA bands and high throughput electrophoresis. It also offers a range of quality control steps and custom databases for efficient sample tracking, storage and retrieval.

Robot 1

Gene assembly, PCR, DNA size selection, cherry picking & quality control
 

Robot 2

Colony picking, culture plating, sample collection, inoculation, & analyte purification